NOTE: This test is for Gram-negative organisms.
The oxidase test determines whether a microbe can oxidize certain aromatic amines, for example, p -aminodimethylaniline, to form colored end products. This oxidation correlates with the cytochrome oxidase activity of some bacteria, including the genera Pseudomonas and Neisseria. While a positive oxidase test is important in the identification of these genera, the test is also useful in characterizing the enteric bacteria ( Enterobacteriaceae), which are oxidase-negative.
NOTE: You must use bacteria from a culture grown on T-soy agar for 24 - 36 hours. Cultures which are too young or too old may give inaccurate results.
Procedure: (This reagent is a liquid in an ampule.)
Modified Oxidase Test (Microdase Disks)
NOTE: This test is for Gram-positive, catalase-positive cocci, only. You must use bacteria from a culture grown on Blood agar for 24 - 36 hours. Cultures which are too young or too old may give inaccurate results.
This test is used for differentiating Micrococcus from Staphylococcus . Micrococci should yield a positive result. Staphylococci should yield a negative result, with the exception of Staphylococcus sciuri.
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Last Modified: Thursday, 02-Jul-1998 00:00:00 CDT