Gel Permeation Chromatography
[Allcock-394]
- No equation for Gel Permeation Chromatography at this time.
- For GPC work to be reproducible you must have control over:
- flow rate
- temperature
- solvent composition
- tunnel size of the stationary particles (it must not change)
- other reasons may be added later...
- Problems to consider:
- Your sample polymer must be soluble in a suitable solvent.
If polystyrene is insoluble in your solvent, and your stationary
phase is crosslinked polystyrene, the pores may close and no separation
based on size will take place. Suitable solvents include tetrahydrofuran,
benzene, xylene, chloroform, dimethylformamide, and fluorinated
alcohols.
- You must filter the solvent you use to dissolve your sample before
you run it through the GPC, because stray debris (e.g. polymers from
dead skin cells, fibers from your clothes, etc.) would register on
the detector as sample.
- The Universal Calibration Equation is part of the explanation
for why GPC works, so it is included below:
- The
Universal constant
,
is
interesting because it ties together viscosity, molecular weight, and
coil dimension.
Last Update- January 8, 1995- wld