1. With sample chamber empty, set desired wavelength then adjust to 0%T with left knob on front panel.

2. Insert blank solution, close lid and adjust 100%T
with right knob on front panel.

3. Insert dye solutions, read and record %T values.

4. Change wavelength, repeat steps 2-4.
NOTE:  Some digital instruments have a filter on the lower left of the front panel that must be changed midway through the wavelength range studied.
Spectronic 20 Instructions
(available next to each instrument)