Original Chromatography Experiment
Later
Start: A glass
column is filled
with powdered
limestone
(CaCO3).
End: A series of colored bands is
seen to form,
corresponding to
the different pigments in the original plant extract. These bands were later determined to be chlorophylls, xanthophylls and carotenoids.
An EtOH extract
of leaf pigments
is applied to the
top of the column.
EtOH is used to
flush the pigments
down the column.