Chem2 Labs WS/96 Gas Chromatography Postlab 1. If I inject 0.50 ml of pure CHCl3 vapor into a GC at 25.0 oC and 1.00 atm pressure, what mass of material am I injecting? (R=0.082 L atm/mole K, MWt of CHCl3 = 119.4 gm/mole) 2. What effect would failure to completely fill the column with Tide have on the efficiency of separation of the GC? Why? 3. Would it be possible to detect compounds not containing a halogen (Cl, Br, I) using a Beilstein detector? Explain. 4. The number of theoretical plates, N, is a measure of efficiency of a GC column. Calculate the peak widths, wB, for a substance run on two different columns, one with N=70.0, the other with N=7000., assuming the retention time, tR, is 100.0 sec for both columns. N=16(tR/wB)2 5. Calculate how the increase in N will affect the peak height, H, for the substance above if identical amounts (Areas) are injected into the two different columns? A=1/2 wB(H)